TGFA Antikörper (AA 24-98)

Produktnummer ABIN1860726

Produktdetails anti-TGFA Antikörper

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Bindungsspezifität: AA 24-98

Reaktivität: Kaninchen

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequenz: MGHHHHHHSG SEF- ENSTSPL SDPPVAAAVV SHFNDCPDSH TQFCFHGTCR FLVQEDKPAC VCHSGYVGAR CEHADLLAVV AASQKKQA

Spezifität: It has been selected for its ability to recognize TGFa in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant TGFa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TGFa.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant TGFa (Glu24~Ala98) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: Transforming Growth Factor Alpha (TGFA Produkte)

Synonyme: TFGA antikoerper, LOC100009482 antikoerper, TGF alpha antikoerper, TGFA antikoerper, wa-1 antikoerper, wa1 antikoerper, RATTGFAA antikoerper, TGFAA antikoerper, NV15606 antikoerper, si:ch211-10e8.7 antikoerper, transforming growth factor alpha antikoerper, transforming growth factor alpha L homeolog antikoerper, transforming growth factor-alpha antikoerper, transforming growth factor, alpha antikoerper, TGFA antikoerper, TGF-A antikoerper, Tgfa antikoerper, tgfa.L antikoerper, LOC100121300 antikoerper, LOC100009150 antikoerper, tgfa antikoerper

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway