PLK4 Antikörper (N-Term)

Produktnummer ABIN184701

Produktdetails anti-PLK4 Antikörper

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PLK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Sak / STK18 / PLK4

Sequenz: ATCIGEKIED FKVGN

Homologie: Human, Dog, Cow

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Approx 110 kDa band observed in Human Colon lysates (calculated MW of 109 kDa according to NP_055079.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PLK4

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Andere Bezeichnung: PLK4 (PLK4 Produkte)

Synonyme: CG7186 antikoerper, DmSAK antikoerper, Dmel\\CG7186 antikoerper, PLK4 antikoerper, Plk4 antikoerper, SAK/PLK antikoerper, SAK/PLK4 antikoerper, Sak antikoerper, plk4 antikoerper, sak antikoerper, SAK antikoerper, STK18 antikoerper, 1700028H20 antikoerper, AI385771 antikoerper, Stk18 antikoerper, PLK-4 antikoerper, stk18 antikoerper, wu:fi33g07 antikoerper, Sak kinase antikoerper, polo like kinase 4 antikoerper, polo-like kinase 4 antikoerper, polo-like kinase 4 (Drosophila) antikoerper, serine/threonine-protein kinase PLK4 antikoerper, SAK antikoerper, PLK4 antikoerper, Plk4 antikoerper, plk4 antikoerper, LOC5571876 antikoerper

Substanzklasse: Phage Protein

Hintergrund: PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase, STK18, serine/threonine protein kinase SAK, MCCRP2, STK 18

Gen-ID: 10733

NCBI Accession: NP_055079, NP_001177728

Pathways: M Phase