alpha Tubulin Antikörper (TUBA1) (AA 419-435)

Details for Product anti-TUBA1 Antibody No. ABIN1742547, Anbieter: Anmelden zum Anzeigen
Antigen
  • Tuba1
  • K-ALPHA-1
  • TUBA2
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • mec-12
  • tuba4
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 1b
  • tubulin alpha 4a
  • tubulin-alpha
  • tubulin alpha 4b L homeolog
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • TUBA3E
  • tuba1b
  • TUBA4A
  • LOC100101229
  • tuba4b.L
  • Tuba1b
Epitop
AA 419-435
30
18
16
10
7
6
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Invertebrat, Maus, Ratte (Rattus), Vertebrate, Yeast (Saccharomyces cerevisiae)
261
153
140
63
56
56
46
32
31
29
29
29
29
28
27
26
19
19
19
17
9
8
8
8
8
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Wirt
Maus
164
108
27
5
2
2
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser alpha Tubulin Antikörper ist unkonjugiert
14
11
8
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
267
101
90
89
88
86
54
39
32
13
11
8
4
4
2
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Klon 3A2
Isotyp IgG1
Spezifität Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
Reinigung purified IgG. Azide was added before lyophilization.
Andere Bezeichnung alpha-Tubulin (TUBA1 Antibody Abstract)
Forschungsgebiet Cytoskeleton
Pathways Microtubule Dynamics
Applikationshinweise WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200
Kommentare

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Lagerung -20 °C
Informationen zur Lagerung Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bilder des Herstellers
Immunocytochemistry (ICC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) anti-alpha Tubulin (TUBA1) (AA 419-435) antibody
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). N...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) dilution: 1 : 1000, sample: rat brain homogenate
Produkt verwendet in: Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, 2015 (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014 (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, 2013 (PubMed).

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