alpha Tubulin Antikörper (TUBA1) (AA 419-435)

Details for Product anti-TUBA1 Antibody No. ABIN1742547, Anbieter: Anmelden zum Anzeigen
Antigen
  • Tuba1
  • K-ALPHA-1
  • TUBA2
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • mec-12
  • tuba4
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 1b
  • tubulin alpha 4a
  • tubulin-alpha
  • tubulin alpha 4b L homeolog
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • TUBA3E
  • tuba1b
  • TUBA4A
  • LOC100101229
  • tuba4b.L
  • Tuba1b
Epitop
AA 419-435
33
19
12
11
9
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Invertebrat, Maus, Ratte (Rattus), Vertebrate, Yeast (Saccharomyces cerevisiae)
290
166
159
60
52
51
46
32
31
29
29
29
26
26
25
24
19
19
19
17
9
8
8
8
8
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Maus
164
141
26
5
2
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser alpha Tubulin Antikörper ist unkonjugiert
17
12
10
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
294
128
107
97
96
89
56
39
35
31
13
8
4
2
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Klon 3A2
Isotyp IgG1
Spezifität Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
Reinigung purified IgG. Azide was added before lyophilization.
Plasmids, Primers & others Plasmide, Primers & weitere alpha Tubulin products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung alpha-Tubulin (TUBA1 Antibody Abstract)
Pathways Microtubule Dynamics
Applikations-hinweise WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200
Kommentare

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Lagerung -20 °C
Informationen zur Lagerung Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bilder des Herstellers
Immunocytochemistry (ICC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) anti-alpha Tubulin (TUBA1) (AA 419-435) antibody
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). N...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) dilution: 1 : 1000, sample: rat brain homogenate
Produkt verwendet in: Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, 2015 (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014 (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, 2013 (PubMed).

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