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GFP Antikörper (AA 183-191)

Name: GFP Antikörper (AA 183-191)
SKU: ABIN1742292
Price: 462.85 EUR
Availability: InStock

GFP Reaktivität: Aequorea victoria WB Wirt: Maus Monoclonal 101G4 unconjugated

dilution: 1 : 1000, sample: recombinant protein (GFP Antikörper (AA 183-191))

1 image

(3 Referenzen)

Produktnummer ABIN1742292

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Bindungsspezifität
16

15

10

9

5

4

3

3

2

2

2

2

1

1

1

1
AA 183-191
Reaktivität
177

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Wirt
74

72

35

11

9

6

5

1
Maus
Passende Sekundärantikörper
Klonalität
121

87
Monoklonal
Konjugat
104

14

12

9

6

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
172

87

56

42

33

32

31

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB)

Spezifität

Specific for GFP of jelly-fish.

Aufreinigung

purified IgG. Azide was added before lyophilization.

Immunogen

Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)

Klon

101G4

Isotyp

IgG2b

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Applikationshinweise

WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet

Kommentare

ABIN1742292 is recommended for IP and ICC.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer

PBS, 0.02% sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung

-20 °C

Informationen zur Lagerung

Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, (2013) (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).

Kaddai, Gonzalez, Keslair, Grémeaux, Bonnafous, Gugenheim, Tran, Gual, Le Marchand-Brustel, Cormont: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, (2009) (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Synonyme

green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse

Viral Protein