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GFP Antikörper

Name: GFP Antikörper
Brand: antibodies-online
SKU: ABIN7141875
Price: 220 EUR
Availability: InStock

GFP WB, ELISA, IF, IP, FACS Wirt: Maus Monoclonal 6C11C11 unconjugated

Western Blot Positive WB detected in: 50 ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5 min

Immunofluorescence staining of 293F cells transfected with GFP with ABIN7141875 at 1:100, counter-stained with DAPI.

Western Blot Positive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50 ng, 5, 293F whole cell lysate transfected with GFP for 5 μg All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1 min

Overlay histogram showing 293F cells transfected with GFP stained with ABIN7141875 (red line) at 1:200.

FACS

Western Blot Positive WB detected in: Recombinant protein at 25 ng, 12.

Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with ABIN7141875 at 1:200.

FACS

Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP Lane 1: Mouse control IgG2b instead of ABIN7141875 in 293F whole cell lysate transfected with GFP Lane 2: ABIN7141875 (4 μg) + 293F whole cell lysate transfected with GFP (500 μg) Lane 3: 293F whole cell lysate transfected with GFP (5 μg) For western blotting, the blot was detected with ABIN7141875 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

7 Abbildungen

Produktnummer ABIN7141875

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))

Reaktivität

Bitte anfragen
Wirt
74

72

35

11

9

6

5

1
Maus
Passende Sekundärantikörper
Klonalität
121

87
Monoklonal
Konjugat
104

14

12

9

6

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
172

86

55

42

32

31

31

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Aufreinigung

Protein G purified

Immunogen

Recombinant GFP Protein

Klon

6C11C11

Isotyp

IgG2b

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Applikationshinweise

Recommended dilution: WB:1:50000-1:6400000, IF:1:50-1:200, FC:1:100-1:300, IP:1 μL-2 μL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Synonyme

green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse

Viral Protein

Hintergrund

Background: The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
Aliases: CFP, eGFP, eYFP, GFP, GFP tag, YFP