Midkine Antikörper

Produktnummer ABIN1499470

Produktdetails anti-Midkine Antikörper

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Midkine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human Midkine

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Midkine.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8221 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Midkine (LS-C104571) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Andere Bezeichnung: MDK / Midkine (MDK Produkte)

Synonyme: ARAP antikoerper, MK antikoerper, NEGF2 antikoerper, Mek antikoerper, MK-B antikoerper, PTF-alpha-1 antikoerper, mdk antikoerper, midkine antikoerper, negf2 antikoerper, RIHB antikoerper, cb518 antikoerper, fb79b05 antikoerper, fj33b02 antikoerper, fj48a12 antikoerper, mdk2 antikoerper, plei2 antikoerper, wu:fb79b05 antikoerper, wu:fj33b02 antikoerper, wu:fj48a12 antikoerper, MK-A antikoerper, PTF-alpha-2 antikoerper, X-PTF-alpha1 antikoerper, XMK antikoerper, mdk-a antikoerper, mdk-b antikoerper, midkine antikoerper, midkine L homeolog antikoerper, midkine (neurite growth-promoting factor 2) antikoerper, midkine b antikoerper, midkine S homeolog antikoerper, MDK antikoerper, Mdk antikoerper, mdk.L antikoerper, mdkb antikoerper, mdk.S antikoerper

Hintergrund: Name/Gene ID: MDK

Synonyms: MDK, ARAP, MK, MK1, NEGF2, Midkine, Retinoic acid inducible factor

Gen-ID: 4192

UniProt: P21741

Pathways: RTK Signalweg, M Phase, Skeletal Muscle Fiber Development