RFC1 Antikörper (AA 401-500) (Biotin)

Produktnummer ABIN1390477

Produktdetails anti-RFC1 Antikörper (Biotin)

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Bindungsspezifität: AA 401-500

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFC1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus

Homologie: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RFC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu RFC1

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Andere Bezeichnung: RFC1 (RFC1 Produkte)

Synonyme: A1 antikoerper, MHCBFB antikoerper, PO-GA antikoerper, RECC1 antikoerper, RFC antikoerper, RFC140 antikoerper, AtRFC1 antikoerper, replication factor C 1 antikoerper, replication factor C1 antikoerper, 140kDa antikoerper, Alp145 antikoerper, Recc1 antikoerper, Replication factor C1 antikoerper, si:dkey-170e5.1 antikoerper, zgc:162875 antikoerper, replication factor C subunit 1 antikoerper, replication factor C1 antikoerper, replication factor C (activator 1) 1 antikoerper, DNA replication factor C complex subunit Rfc1 antikoerper, RFC1 antikoerper, Rfc1 antikoerper, rfc1 antikoerper

Hintergrund:

Synonyms: DNA binding Protein PO GA, DNA-binding protein PO-GA, MHC binding factor beta, MHCBFB, RECC1, Replication factor C 140 kDa subunit, Replication factor C, Replication factor C large subunit, Replication factor C subunit 1, Replication factor C1, RF-C 140 kDa subunit, RFC1, RFC1_HUMAN, RFC140, RFC140 Replication Factor C 140 kDa subunit, A1 140 kDa subunit, A1 P145 Activator 1 large subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1.

Background: Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (including RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC1 is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during FAS-mediated apoptosis. In addition, phosphorylation of the PCNA binding domain of RFC1 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibits DNA synthesis. The human RFC1 gene maps to chromosome 4p14 and encodes the RFC1 subunit.

Gen-ID: 5981

UniProt: P35251

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport