Cyclin B1 Antikörper (pSer126)

Produktnummer ABIN129589

Produktdetails anti-Cyclin B1 Antikörper

Target: Cyclin B1 (CCNB1)

Bindungsspezifität: AA 120-131, pSer126

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität: Ratte (Rattus), Hund, Schimpanse, Maus, Hamster

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.01 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Cyclin B1 Antikörper (ABIN129589)

Geng, Zhang, Zheng, Legerski: "Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." in: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1 (CCNB1 Produkte)

Synonyme: ccnb antikoerper, cycb antikoerper, cb267 antikoerper, cycb1 antikoerper, wu:fa19g04 antikoerper, wu:fb16d01 antikoerper, wu:fb16e07 antikoerper, wu:fi21c01 antikoerper, ccnb1 antikoerper, MGC53596 antikoerper, CCNB antikoerper, Ccnb1-rs1 antikoerper, Ccnb1-rs13 antikoerper, CycB1 antikoerper, Cycb-4 antikoerper, Cycb-5 antikoerper, Cycb1-rs1 antikoerper, cyclin B1 antikoerper, cyclin B1 S homeolog antikoerper, ccnb1 antikoerper, ccnb1.2 antikoerper, ccnb1.S antikoerper, CCNB1 antikoerper, Ccnb1 antikoerper, ccnb1.2.S antikoerper

Hintergrund: Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.  S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex.  Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Synonyms: Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase