ErbB2/Her2 Antikörper (pSer1113)

Produktnummer ABIN126790

Produktdetails anti-ErbB2/Her2 Antikörper

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Bindungsspezifität: pSer1113

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ErbB2/Her2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Spezifität: This antibody specifically recognizes erbB2 phosphorylated at Serine 1113 at 185 kDa.

Produktmerkmale: Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2

Aufreinigung: Subsequent Thiophilic and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Phosphopetide conjugated to KLH. Epitope: Phospho-Serine 1113AA Sequence: Q R Y pS E D P

Klon: 9-00E-010

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: Use at 0.1 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunocytochemistry: Use 1-10 μg/mLIncluded Positive Control: Cell lysate from untreated pervanadate-treated A431 cells (SeeProtocols for more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from A 431 pervanadate treatedDescription: Cell lysate from pervanadate-treated A431 cells, epidermoid carcinoma(Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS containing 0.09 % Sodium Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: CD340 / ERBB2 / HER2 (ErbB2/Her2 Produkte)

Synonyme: CD340 antikoerper, HER-2 antikoerper, HER-2/neu antikoerper, HER2 antikoerper, MLN 19 antikoerper, NEU antikoerper, NGL antikoerper, TKR1 antikoerper, Erbb-2 antikoerper, Neu antikoerper, c-erbB2 antikoerper, c-neu antikoerper, mKIAA3023 antikoerper, wu:fv70f10 antikoerper, zgc:63601 antikoerper, erb2 antikoerper, erb-b2 receptor tyrosine kinase 2 antikoerper, ERBB2 antikoerper, Erbb2 antikoerper, erbb2 antikoerper

Hintergrund: ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of erbB2 at serine 1113 has been correlated with erbB2 overexpression and a poor prognosis. Thus the phosphorylation status of serine 1113 in erbB2 represents a novel and informative biomarker of cancer cell biology and tumor behavior. (Mol. Cell Biochem. 218: 47-54, 2001).Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2

Gen-ID: 2064

UniProt: P04626

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development