Cyclin B1 Antikörper

Produktnummer ABIN121517

Produktdetails anti-Cyclin B1 Antikörper

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 62 kDa Cyclin B1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin B1 from other species may also occur.

Aufreinigung: Delipidation and defibrination.

Immunogen: Full length GST fusion protein corresponding to the human cyclin B gene

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA (1/1,000-1/2,000),Immuno-precipitation and Westernblot (1/500- 1/1,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85 mg/mL (by Refractometry)

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 °C or below. Avoid repeated freezing and thawing.
Shelf life one year from despatch.

Haltbarkeit: 12 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1 (CCNB1 Produkte)

Synonyme: ccnb antikoerper, cycb antikoerper, cb267 antikoerper, cycb1 antikoerper, wu:fa19g04 antikoerper, wu:fb16d01 antikoerper, wu:fb16e07 antikoerper, wu:fi21c01 antikoerper, ccnb1 antikoerper, MGC53596 antikoerper, CCNB antikoerper, Ccnb1-rs1 antikoerper, Ccnb1-rs13 antikoerper, CycB1 antikoerper, Cycb-4 antikoerper, Cycb-5 antikoerper, Cycb1-rs1 antikoerper, cyclin B1 antikoerper, cyclin B1 S homeolog antikoerper, ccnb1 antikoerper, ccnb1.2 antikoerper, ccnb1.S antikoerper, CCNB1 antikoerper, Ccnb1 antikoerper, ccnb1.2.S antikoerper

Hintergrund: In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase