CD45 Antikörper
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- Target Alle CD45 (PTPRC) Antikörper anzeigen
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reaktivität
- Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD45 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- This antibody is directed against a high molecular weight band of the leucocyte common antigen (LCA). MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes.
- Aufreinigung
- Affinity chromatography on Protein G
- Immunogen
- Purified spleen leucocyte common antigen. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line.
- Klon
- OX-33
- Isotyp
- IgG1
- Top Product
- Discover our top product PTPRC Primärantikörper
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- Applikationshinweise
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Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine- paraformaldehyde) - refer to ref. Whiteland, J.L. et al. (1995) - reference 4.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Andere Bezeichnung
- CD45 / LCA (PTPRC Produkte)
- Synonyme
- PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper
- Hintergrund
- CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
- Gen-ID
- 24699
- NCBI Accession
- NP_001103357
- UniProt
- P04157
- Pathways
- T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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