CD45 Antikörper

Produktnummer ABIN119550

Produktdetails anti-CD45 Antikörper

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reaktivität: Human, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Kreuzreaktivität (Details): Species reactivity (tested):Human, non-Human Primates.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Human thymocytes and T lymphocytes

Klon: MEM-55

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (FFPE sections). No pre-treatment of tissue sections is essential.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the original vial at 2-8 °C. Freeze stock solution or aliquots thereof at -20 °C.

Referenzen für anti-CD45 Antikörper (ABIN119550)

de Torre-Minguela, Barberà-Cremades, Gómez, Martín-Sánchez, Pelegrín: "Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process." in: Scientific reports, Vol. 6, pp. 22586, (2017) (PubMed).

Honda, Yasuda, Tanaka, Hagiwara, Arai, Yoshino: "Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticum AMB-1 by using a magnetosome display system." in: Applied and environmental microbiology, Vol. 81, Issue 4, pp. 1472-6, (2016) (PubMed).

Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).

Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

Curnis, Gasparri, Sacchi, Cattaneo, Magni, Corti: "Targeted delivery of IFNgamma to tumor vessels uncouples antitumor from counterregulatory mechanisms." in: Cancer research, Vol. 65, Issue 7, pp. 2906-13, (2005) (PubMed).

Reynaud, Bruno, Boullanger, Grange, Barbesti, Niveleau: "Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies." in: Cancer letters, Vol. 61, Issue 3, pp. 255-62, (1992) (PubMed).

Details zu CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45 / LCA (PTPRC Produkte)

Synonyme: PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper

Hintergrund: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gen-ID: 5788

NCBI Accession: NP_002829

UniProt: P08575

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling