CD45 Antikörper

Produktnummer ABIN119549

Produktdetails anti-CD45 Antikörper

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reaktivität: Human, Kaninchen, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes. Clone MEM-55 immunoprecipitates bands of 190, 200 and 205 kDa.

Keine Kreuzreaktivität: Pferd, Ziege, Llama

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rabbit, Rhesus Monkey, Monkey.

Aufreinigung: Affinity chromatography on Protein A

Klon: MEM-55

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/50 - 1/200, this product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45 / LCA (PTPRC Produkte)

Synonyme: PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper

Hintergrund: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gen-ID: 5788

NCBI Accession: NP_002829

UniProt: P08575

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling