SUMO3 Antikörper

Produktnummer ABIN118003

Produktdetails anti-SUMO3 Antikörper

Target: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUMO3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Details zu SUMO3

Target: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Andere Bezeichnung: SUMO3 / SMT3A (SUMO3 Produkte)

Synonyme: SMT3A antikoerper, SMT3H1 antikoerper, SUMO-3 antikoerper, Smt3B antikoerper, 2810014B19Rik antikoerper, AA409334 antikoerper, AW121497 antikoerper, AW536077 antikoerper, D10Ertd345e antikoerper, Smt3h1 antikoerper, smt3a antikoerper, smt3h1 antikoerper, fa94a11 antikoerper, im:7141764 antikoerper, sumo2 antikoerper, sumo3 antikoerper, sumo3l antikoerper, wu:fa94a11 antikoerper, wu:fb55d09 antikoerper, zgc:65847 antikoerper, zgc:85643 antikoerper, fc66f10 antikoerper, fl11h12 antikoerper, wu:fc66f10 antikoerper, wu:fl11h12 antikoerper, zgc:86902 antikoerper, SUMO3 antikoerper, ATSUMO3 antikoerper, MCO15.12 antikoerper, MCO15_12 antikoerper, SMALL UBIQUITIN-LIKE MODIFIER 3 antikoerper, SUM3 antikoerper, SUMO 3 antikoerper, small ubiquitin-like modifier 3 antikoerper, GMP1 antikoerper, PIC1 antikoerper, SENTRIN antikoerper, SMT3 antikoerper, SMT3H3 antikoerper, SMTP3 antikoerper, SUMO-1 antikoerper, Smt3C antikoerper, Ubl1 antikoerper, small ubiquitin-like modifier 3 antikoerper, small ubiquitin-like modifier 3 L homeolog antikoerper, small ubiquitin-like modifier 3a antikoerper, small ubiquitin-like modifier 3b antikoerper, small ubiquitin-related modifier 2 pseudogene antikoerper, SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antikoerper, small ubiquitin-like modifier 2 antikoerper, small ubiquitin-like modifier 1 antikoerper, SUMO3 antikoerper, Sumo3 antikoerper, sumo3.L antikoerper, sumo3a antikoerper, sumo3b antikoerper, LOC490297 antikoerper, sumo3 antikoerper, sumo2 antikoerper, Sumo1 antikoerper

Hintergrund: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to human. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c- Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IκB-α, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50 % sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18 % homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with β-sheets wrapped around an α-helix.Synonyms: SMT3 homolog 1, SMT3H1, SUMO-3, Small ubiquitin-related modifier 3, Ubiquitin-like protein SMT3A

Gen-ID: 6612

NCBI Accession: NP_008867

UniProt: P55854