LAT Antikörper (Linker For Activation of T Cells) (pTyr171) (Alexa Fluor 647)

Details for Product anti-LAT Antibody No. ABIN1177077, Anbieter: Anmelden zum Anzeigen
Antigen
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Epitop
pTyr171
31
25
22
22
14
6
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
277
122
103
3
2
1
Wirt
Maus
168
102
13
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser LAT Antikörper ist konjugiert mit Alexa Fluor 647
9
8
8
7
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4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Applikation
Intracellular Staining (ICS)
214
81
51
50
39
33
32
10
5
3
2
2
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Phosflow™
Immunogen Phosphorylated Human LAT
Klon I58-1169
Isotyp IgG1, kappa
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmide, Primers & weitere LAT products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung LAT (LAT Antibody Abstract)
Hintergrund Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development. The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Produkt verwendet in: Cho, Velikovsky, Swaminathan, Houtman, Samelson, Mariuzza: "Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads." in: The EMBO journal, Vol. 23, Issue 7, pp. 1441-51, 2004 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, 2002 (PubMed).

Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, 2002 (PubMed).

Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, 2001 (PubMed).

Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, 2001 (PubMed).

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