COL6 Antikörper (Biotin)

Produktnummer ABIN116565

Produktdetails anti-COL6 Antikörper (Biotin)

Target: COL6 (Collagen, Type VI (COL6))

Reaktivität: Human, Rind (Kuh), Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL6 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule .

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Collagen type VI purified from Human and Bovine placenta.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Collagen VI at a dilution of 1: 5,000 to 1: 10,000. This product was alsoassayed against 1.0 μg of Collagen VI in a standard sandwich ELISA using PeroxidaseConjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the stock concentration is suggested for this product. ForImmunohistochemistry on frozen tissue sections dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent)

Konzentration: 1.0 mg/mL (by UV absorbance at 280)

Buffer: 0.125 M Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (Immunoglobulin and Protease free)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.

Details zu COL6

Target: COL6 (Collagen, Type VI (COL6))

Abstract: COL6 Produkte

Hintergrund: The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.Synonyms: COL6A1

Gen-ID: 1291

NCBI Accession: NP_001839

UniProt: P12109