COL6 Antikörper

Produktnummer ABIN462457

Produktdetails anti-COL6 Antikörper

Target: COL6 (Collagen, Type VI (COL6))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser COL6 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Dot Blot (DB)

Marke: IHC-plus™

Spezifität: Reacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10 % cross-reactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e. g., laminin, fibronectin).

Aufreinigung: Immunoaffinity purified

Immunogen: Human type VI collagen.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, ICC (1:10 - 1:20), IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu COL6

Target: COL6 (Collagen, Type VI (COL6))

Andere Bezeichnung: Collagen VI (COL6 Produkte)