GFAP Antikörper

Produktnummer ABIN112031

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-GFAP Antikörper wird verwendet zum Nachweis von GFAP in Proben von Human. Er wurde validiert für WB, IF, IHC (fro) und EIA.

Kurzübersicht für GFAP Antikörper (ABIN112031)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: GF-05

Produktdetails anti-GFAP Antikörper

Spezifität: The antibody recognizes GFAP. It may react with GFAP from other species.

Kreuzreaktivität (Details): Species reactivity (expected):Macaque.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Native

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (has been used with antigen coated at 5 μg/mL, endpoint titer of a 1 mg/mL solutionof this antibody is around 1/500,000). Western blot (recognizes 38-45 kDa neurofilaments). Immunohistochemistry on Frozen Sections and cell smears (0.1-10 μg per slide may beused as a guide). Immunflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-GFAP Antikörper (ABIN112031)

Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.Synonyms: Glial Fibrillary Acidic Protein

Gen-ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136