GFAP
Reaktivität: Human
WB, IHC, IF
Wirt: Kaninchen
Monoclonal
unconjugated
Applikationshinweise
ELISA (has been used with antigen coated at 5 μg/mL, endpoint titer of a 1 mg/mL solutionof this antibody is around 1/500,000). Western blot (recognizes 38-45 kDa neurofilaments). Immunohistochemistry on Frozen Sections and cell smears (0.1-10 μg per slide may beused as a guide). Immunflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.Synonyms: Glial Fibrillary Acidic Protein