POLI Antikörper (Middle Region, N-Term)

Produktnummer ABIN1107000

Produktdetails anti-POLI Antikörper

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Bindungsspezifität: Middle Region, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity column

Immunogen: Synthetic peptide directed towards the N-terminal to middle region of human POLI

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8 °C for one month or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Details zu POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Andere Bezeichnung: DNA Polymerase iota (POLI Produkte)

Synonyme: poli antikoerper, wu:fc27h11 antikoerper, zgc:110185 antikoerper, GB12093 antikoerper, NV16187 antikoerper, RAD30B antikoerper, RAD3OB antikoerper, Rad30b antikoerper, DNA polymerase iota antikoerper, polymerase (DNA directed) iota antikoerper, polymerase (DNA directed), iota antikoerper, POLI antikoerper, poli antikoerper, LOC551219 antikoerper, AFUA_4G11140 antikoerper, LOC5568276 antikoerper, Pol-iota antikoerper, Poli antikoerper

Hintergrund: DNA polymerase iota (POLI) is an error-prone DNA polymerase specifically involved in DNA repair. POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. POLI favors Hoogsteen base-pairing in the active site. POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. POLI may play a role in hypermutation of immunogobulin genes. POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.Synonyms: Eta2, POLI, RAD30 homolog B, RAD30B

Molekulargewicht: POLI: 83 kDa

Gen-ID: 11201

NCBI Accession: NM_007195

Pathways: DNA Reparatur