IL1RN Antikörper

Produktnummer ABIN1102536

Produktdetails anti-IL1RN Antikörper

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1RN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Marke: IHC-plus™

Spezifität: Human IL-1RA

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-1RA.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (2.5 - 3.7 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7435 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-1RA (50 ng/mL) and Human IL-1alpha (50 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required. ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (LS-C104474) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Andere Bezeichnung: IL1RN (IL1RN Produkte)

Synonyme: DIRA antikoerper, ICIL-1RA antikoerper, IL-1RN antikoerper, IL-1ra antikoerper, IL-1ra3 antikoerper, IL1F3 antikoerper, IL1RA antikoerper, IRAP antikoerper, MVCD4 antikoerper, IRAP1 antikoerper, IL1RN antikoerper, IL-1RA antikoerper, F630041P17Rik antikoerper, interleukin 1 receptor antagonist antikoerper, interleukin 1 receptor antagonist-like antikoerper, IL1RN antikoerper, Il1rn antikoerper, IL1RNL antikoerper

Hintergrund: Name/Gene ID: IL1RN
Family: IL-1

Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3

Gen-ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signalweg, Hormone Transport, Cancer Immune Checkpoints