KCNH2 Antikörper (Internal Region)
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- Target Alle KCNH2 Antikörper anzeigen
- KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Ratte, Maus, Hund, Kaninchen, Rind (Kuh), Pferd, Schwein, Affe, Hamster
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser KCNH2 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human KCNH2 / Kv11.1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Bat, Guinea pig (94%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from internal region of human KCNH2 / Kv11.1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Bat, Guinea pig (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product KCNH2 Primärantikörper
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- Applikationshinweise
- Approved: IHC, IHC-P (5 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))
- Andere Bezeichnung
- KCNH2 / HERG (KCNH2 Produkte)
- Synonyme
- ERG1 antikoerper, HERG antikoerper, HERG1 antikoerper, Kv11.1 antikoerper, LQT2 antikoerper, SQT1 antikoerper, erg antikoerper, KCNH2 antikoerper, ERG antikoerper, gp-erg antikoerper, cerg antikoerper, derg antikoerper, erg1 antikoerper, AI326795 antikoerper, LQT antikoerper, Lqt2 antikoerper, M-erg antikoerper, Merg1 antikoerper, merg1a antikoerper, merg1b antikoerper, potassium voltage-gated channel subfamily H member 2 antikoerper, potassium voltage-gated channel, subfamily H (eag-related), member 6a antikoerper, potassium voltage-gated channel, subfamily H (eag-related), member 2 antikoerper, potassium voltage-gated channel subfamily H member 6 antikoerper, KCNH2 antikoerper, kcnh6a antikoerper, Kcnh2 antikoerper, KCNH6 antikoerper
- Hintergrund
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Name/Gene ID: KCNH2
Subfamily: Potassium channel - Eag-related
Family: Ion Channel
Synonyms: KCNH2, Eag homolog, HERG-1, ERG1, HERG, KV11.1, M-erg1, H-ERG, HERG1, SQT1, LQT2, Merg1, Eag-related protein 1, ERG-1 - Gen-ID
- 3757
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