KCNH2 Antikörper (Internal Region)

Produktnummer ABIN1048984

Produktdetails anti-KCNH2 Antikörper

Target: KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KCNH2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human KCNH2 / Kv11.1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%) Elephant, Dog, Horse, Rabbit, Guinea pig (94%) Hamster (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from internal region of human KCNH2 / Kv11.1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%), Elephant, Dog, Horse, Rabbit, Guinea pig (94%), Hamster (82%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu KCNH2

Target: KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))

Andere Bezeichnung: KCNH2 / HERG (KCNH2 Produkte)

Synonyme: ERG1 antikoerper, HERG antikoerper, HERG1 antikoerper, Kv11.1 antikoerper, LQT2 antikoerper, SQT1 antikoerper, erg antikoerper, KCNH2 antikoerper, ERG antikoerper, gp-erg antikoerper, cerg antikoerper, derg antikoerper, erg1 antikoerper, AI326795 antikoerper, LQT antikoerper, Lqt2 antikoerper, M-erg antikoerper, Merg1 antikoerper, merg1a antikoerper, merg1b antikoerper, potassium voltage-gated channel subfamily H member 2 antikoerper, potassium voltage-gated channel, subfamily H (eag-related), member 6a antikoerper, potassium voltage-gated channel, subfamily H (eag-related), member 2 antikoerper, potassium voltage-gated channel subfamily H member 6 antikoerper, KCNH2 antikoerper, kcnh6a antikoerper, Kcnh2 antikoerper, KCNH6 antikoerper

Hintergrund: Name/Gene ID: KCNH2
Subfamily: Potassium channel - Eag-related
Family: Ion Channel

Synonyms: KCNH2, Eag homolog, HERG-1, ERG1, HERG, KV11.1, M-erg1, H-ERG, HERG1, SQT1, LQT2, Merg1, Eag-related protein 1, ERG-1

Gen-ID: 3757