EDNRB Antikörper (N-Term)

Produktnummer ABIN1048557

Produktdetails anti-EDNRB Antikörper

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Bindungsspezifität: N-Term

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EDNRB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human EDNRB. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PTPN5 (68 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Dog (100%) Gorilla, Gibbon (95%) Monkey (89%) Marmoset (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human EDNRB. Percent identity with other species by BLAST analysis: Human, Dog (100%), Gorilla, Gibbon (95%), Monkey (89%), Marmoset (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (4 - 11 μg/mL), WB

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu EDNRB

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Andere Bezeichnung: EDNRB / Endothelin B Receptor (EDNRB Produkte)

Synonyme: ABCDS antikoerper, ET-B antikoerper, ET-BR antikoerper, ETB antikoerper, ETBR antikoerper, ETRB antikoerper, HSCR antikoerper, HSCR2 antikoerper, WS4A antikoerper, Ednra antikoerper, Etb antikoerper, ETR-b antikoerper, ETb antikoerper, Sox10m1 antikoerper, EDNRB antikoerper, abcds antikoerper, etb antikoerper, etrb antikoerper, hscr antikoerper, hscr2 antikoerper, endothelin receptor type B antikoerper, EDNRB antikoerper, Ednrb antikoerper, ednrb antikoerper

Hintergrund: Name/Gene ID: EDNRB
Subfamily: Endothelin
Family: GPCR

Synonyms: EDNRB, ABCDS, Endothelin B receptor, Etb receptor, ET-B, Et-b receptor, Et-b-r, ET-RB, ETBR, ETRB, Etb-svr, WS4A, Endothelin receptor type B, ET-BR, ETB, HSCR, HSCR2

Gen-ID: 1910

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process