CRHR1 Antikörper (Extracellular Domain)

Produktnummer ABIN1048462

Produktdetails anti-CRHR1 Antikörper

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Hund, Affe, Pferd, Rind (Kuh), Hamster, Schaf, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRHR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Sheep, Hamster, Dog, Bovine, Horse, Pig, Opossum (100%) Elephant, Panda, Bat (94%) Marmoset, Mouse, Rat, Seabass, Stickleback, Pufferfish (89%) Turkey, Chicken, Lizard, Zebrafish (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 1st extracellular domain of human CRF1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Sheep, Hamster, Dog, Bovine, Horse, Pig, Opossum (100%), Elephant, Panda, Bat (94%), Marmoset, Mouse, Rat, Seabass, Stickleback, Pufferfish (89%), Turkey, Chicken, Lizard, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (20 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Andere Bezeichnung: CRFR1 / CRHR1 (CRHR1 Produkte)

Synonyme: CRF-R antikoerper, CRF-R-1 antikoerper, CRF-R1 antikoerper, CRF1 antikoerper, CRFR-1 antikoerper, CRFR1 antikoerper, CRH-R-1 antikoerper, CRH-R1 antikoerper, CRH-R1h antikoerper, CRHR antikoerper, CRHR1L antikoerper, CRHR1f antikoerper, Crhr antikoerper, crhr1 antikoerper, crhr1-A antikoerper, CRHR1 antikoerper, CRHR1-IT1-CRHR1 antikoerper, MGC57346-CRHR1 antikoerper, corticotropin releasing hormone receptor 1 antikoerper, corticotropin releasing hormone receptor 1, gene 2 S homeolog antikoerper, corticotropin-releasing factor receptor 1 antikoerper, LINC02210-CRHR1 readthrough antikoerper, CRHR1 antikoerper, Crhr1 antikoerper, crhr1.2.S antikoerper, LOC610380 antikoerper, LOC100065598 antikoerper, LINC02210-CRHR1 antikoerper, crhr1 antikoerper

Hintergrund: Name/Gene ID: CRHR1
Subfamily: Corticotropin releasing factor
Family: GPCR

Synonyms: CRHR1, CRF-R-1, CRFR, CRHR1f, Crf receptor 1, CRH-R1, CRF-R, CRF1, CRFR-1, Crf receptor type 1, Crf receptor type 1, Crf type 1, CRF-R1, Crf1 receptor, CRFR1, CRH-R-1, CRH-R1h, CRHR, CRHR1L

Gen-ID: 1394

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity