CRHR1 Antikörper (AA 107-117)

Produktnummer ABIN238557

Zitiert in 3+ Publikationen. Der Ziege Polyklonal anti-CRHR1 Antikörper (ABIN238557) detektiert spezifisch CRHR1 in WB, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für CRHR1 Antikörper (AA 107-117) (ABIN238557)

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CRHR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-CRHR1 Antikörper

Bindungsspezifität: AA 107-117

Verwendungszweck: CRHR1 / CRF-R (aa 107 - 117)

Sequenz: NEEKKSKVHY H

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with di

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Expression of the protein seen in the membranes, vesicles and cytoplasm of MCF7 and Neuro2a cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-CRHR1 Antikörper (ABIN238557)

Xie, Penzes, Srivastava: "Exchange protein directly activated by cAMP 2 is required for corticotropin-releasing hormone-mediated spine loss." in: The European journal of neuroscience, Vol. 50, Issue 7, pp. 3108-3114, (2020) (PubMed).

Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).

Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

Details zu CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Andere Bezeichnung: CRHR1

Hintergrund: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor

Gen-ID: 1394, 12921, 58959

NCBI Accession: NP_004373

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity