DPP4 Antikörper (Internal Region)

Produktnummer ABIN1048412

Produktdetails anti-DPP4 Antikörper

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Bindungsspezifität: Internal Region

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPP4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CD26. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FAP (56 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%) Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%) Rabbit (88%) Bat, Dog, Cat (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human CD26. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%), Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%), Rabbit (88%), Bat, Dog, Cat (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1.25 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DPP4

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Andere Bezeichnung: DPP4 / CD26 (DPP4 Produkte)

Synonyme: ADABP antikoerper, ADCP2 antikoerper, CD26 antikoerper, DPPIV antikoerper, TP103 antikoerper, MGC81966 antikoerper, MOP9.8 antikoerper, MOP9_8 antikoerper, DPP4 antikoerper, si:ch73-2d23.3 antikoerper, Cd26 antikoerper, Dpp-4 antikoerper, THAM antikoerper, dipeptidyl peptidase 4 antikoerper, dipeptidyl-peptidase 4 S homeolog antikoerper, prolyl oligopeptidase family protein antikoerper, dipeptidyl-peptidase 4 antikoerper, dipeptidylpeptidase 4 antikoerper, DPP4 antikoerper, dpp4.S antikoerper, AT5G24260 antikoerper, dpp4 antikoerper, Dpp4 antikoerper

Hintergrund: Name/Gene ID: DPP4
Subfamily: Serine S9B
Family: Protease

Synonyms: DPP4, ADCP-2, ADABP, Dipeptidyl peptidase IV, Dipeptidyl-peptidase 4, DPPIV, ADCP2, T-cell activation antigen CD26, CD26, CD26 antigen, Dipeptidyl peptidase 4, Dipeptidylpeptidase 4, DPP IV, TP103

Gen-ID: 1803

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity