Serotonin Receptor 2B Antikörper (Extracellular Domain)

Produktnummer ABIN1048266

Produktdetails anti-Serotonin Receptor 2B Antikörper

Target: Serotonin Receptor 2B (HTR2B)

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 2B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human 5HT2B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Bovine, Bat (93%) Mouse, Hamster, Panda, Horse, Rabbit, Pig (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from 2nd extracellular domain of human 5HT2B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Bovine, Bat (93%), Mouse, Hamster, Panda, Horse, Rabbit, Pig (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 - 6 μg/mL)

Not recommended for: ICC

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Serotonin Receptor 2B

Target: Serotonin Receptor 2B (HTR2B)

Andere Bezeichnung: HTR1B / 5-HT2B Receptor (HTR2B Produkte)

Synonyme: 5-HT2B antikoerper, 5HT2B antikoerper, 5htr2b antikoerper, HTR2B antikoerper, 5ht2b antikoerper, zgc:194094 antikoerper, zgc:194119 antikoerper, AJ012488 antikoerper, AV377389 antikoerper, 5-HT(2B) antikoerper, 5-hydroxytryptamine receptor 2B antikoerper, 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled antikoerper, 5-hydroxytryptamine (serotonin) receptor 2B antikoerper, HTR2B antikoerper, htr2b antikoerper, Htr2b antikoerper

Hintergrund: Name/Gene ID: HTR2B
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR2B, 5-HT 2B receptor, 5HT2B Receptor, 5-HT(2B), 5-HT-2B, 5-HT2b receptor, 5-HT2B, Serotonin 2b receptor, Serotonin 5-HT-2b receptor, Serotonin receptor 2B

Gen-ID: 3357

Pathways: JAK-STAT Signalweg, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process