PTPN11 ELISA Kit
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- Target Alle PTPN11 ELISA Kits anzeigen
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- Bindungsspezifität
- pTyr542
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Reaktivität
- Ratte, Human, Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Human/Mouse/Rat Phospho-SHP2 (Y542) ELISA Kit. This assay semi-quantitatively measures phophorylated SHP2 (Tyr542) in lysate samples.
- Proben
- Cell Lysate, Tissue Lysate
- Analytische Methode
- Semi-Quantitative
- Spezifität
- The antibody pair provided in this kit recognizes human, mouse, and rat SHP2 phosphorylated at site Tyrosine-542
- Produktmerkmale
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Benötigtes Material
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PTPN11 ELISA Kit
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- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Haltbarkeit
- 6 months
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- Target Alle PTPN11 ELISA Kits anzeigen
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- Andere Bezeichnung
- SHP2 (PTPN11 Produkte)
- Synonyme
- BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, 2700084A17Rik ELISA Kit, AW536184 ELISA Kit, PTP1D ELISA Kit, SAP-2 ELISA Kit, SHP-2 ELISA Kit, Shp2 ELISA Kit, Syp ELISA Kit, SYP ELISA Kit, bptp3 ELISA Kit, cfc ELISA Kit, ns1 ELISA Kit, ptp-2 ELISA Kit, ptp2c ELISA Kit, ptpn11 ELISA Kit, ptpn11-a ELISA Kit, ptpn11-b ELISA Kit, shp-2 ELISA Kit, shp2 ELISA Kit, fa14b09 ELISA Kit, wu:fa14b09 ELISA Kit, wu:fi24f03 ELISA Kit, zgc:55388 ELISA Kit, zgc:63553 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 S homeolog ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, a ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, b ELISA Kit, PTPN11 ELISA Kit, Ptpn11 ELISA Kit, ptpn11.S ELISA Kit, ptpn11a ELISA Kit, ptpn11b ELISA Kit
- Substanzklasse
- Viral Protein
- Hintergrund
- SHP2 phosphorylated at Tyrosine-542
- Gen-ID
- 5781
- UniProt
- Q06124
- Pathways
- JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effekt
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