PTPN11 ELISA Kit
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- Target Alle PTPN11 ELISA Kits anzeigen
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- Bindungsspezifität
- pTyr542
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Reaktivität
- Human, Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Cell ELISA
- Applikation
- ELISA
- Verwendungszweck
- Cell-Based Human/Mouse SHP2 (Y542) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
- Proben
- Cell Culture Cells
- Analytische Methode
- Semi-Quantitative
- Spezifität
- The antibodies provided in this kit recognizes human and mouse SHP2 phosphorylated at Tyrosine-542 and total SHP2 for comparison.
- Produktmerkmale
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- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
- Bestandteile
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- uncoated 96-well Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- Benötigtes Material
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Probenmenge
- 100 μL
- Plattentyp
- Uncoated
- Protokoll
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- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacture's instructions.
- Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
- Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 6 months
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- Target Alle PTPN11 ELISA Kits anzeigen
- PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
- Andere Bezeichnung
- SHP2 (PTPN11 Produkte)
- Synonyme
- BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, 2700084A17Rik ELISA Kit, AW536184 ELISA Kit, PTP1D ELISA Kit, SAP-2 ELISA Kit, SHP-2 ELISA Kit, Shp2 ELISA Kit, Syp ELISA Kit, SYP ELISA Kit, bptp3 ELISA Kit, cfc ELISA Kit, ns1 ELISA Kit, ptp-2 ELISA Kit, ptp2c ELISA Kit, ptpn11 ELISA Kit, ptpn11-a ELISA Kit, ptpn11-b ELISA Kit, shp-2 ELISA Kit, shp2 ELISA Kit, fa14b09 ELISA Kit, wu:fa14b09 ELISA Kit, wu:fi24f03 ELISA Kit, zgc:55388 ELISA Kit, zgc:63553 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 S homeolog ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, a ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, b ELISA Kit, PTPN11 ELISA Kit, Ptpn11 ELISA Kit, ptpn11.S ELISA Kit, ptpn11a ELISA Kit, ptpn11b ELISA Kit
- Substanzklasse
- Viral Protein
- Hintergrund
- Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D (PTP-1D), Protein-tyrosine phosphatase 2C (PTP-2C), SH-PTP2 (SHP-2), SH-PTP3
- Gen-ID
- 5781
- UniProt
- Q06124
- Pathways
- JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effekt
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