p21 Antikörper

Produktnummer ABIN966329

Produktdetails anti-p21 Antikörper

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Spezifität: This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).

Aufreinigung: Concentrated.

Immunogen: Human p21 protein.

Klon: B459

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-p21 Antikörper (ABIN966329)

Nakanishi, Robetorye, Adami, Pereira-Smith, Smith: "Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, (1995) (PubMed).

Nakanishi, Robetorye, Pereira-Smith, Smith: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, (1995) (PubMed).

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: WAF 1/p21 Protein (CDKN1A Produkte)

Synonyme: CCND1 antikoerper, CDKN1A antikoerper, CAP20 antikoerper, CDKN1 antikoerper, CIP1 antikoerper, MDA-6 antikoerper, P21 antikoerper, SDI1 antikoerper, WAF1 antikoerper, p21CIP1 antikoerper, CDKI antikoerper, Cdkn1 antikoerper, Waf1 antikoerper, mda6 antikoerper, p21Cip1 antikoerper, p21WAF antikoerper, Cip1 antikoerper, UV96 antikoerper, p21 antikoerper, cip1 antikoerper, p16Xic2 antikoerper, sdi1 antikoerper, waf1 antikoerper, cap20 antikoerper, cdkn1 antikoerper, mda-6 antikoerper, p21cip1 antikoerper, si:ch1073-48m5.2 antikoerper, KRAS2 antikoerper, p21ras antikoerper, cyclin D1 antikoerper, cyclin dependent kinase inhibitor 1A antikoerper, cyclin-dependent kinase inhibitor 1A (P21) antikoerper, cyclin-dependent kinase inhibitor 1A antikoerper, cyclin-dependent kinase inhibitor 1A L homeolog antikoerper, cyclin-dependent kinase inhibitor 1 antikoerper, KRAS proto-oncogene, GTPase antikoerper, CCND1 antikoerper, CDKN1A antikoerper, Cdkn1a antikoerper, cdkn1a.L antikoerper, cki1 antikoerper, cdkn1a antikoerper, KRAS antikoerper

Molekulargewicht: 21 kDa

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie