RFP Antikörper (Red Fluorescent Protein) (Biotin)

Details for Product anti-RFP Antibody No. ABIN401226, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
81
50
36
9
7
2
1
1
Wirt
Kaninchen
96
36
14
9
1
Klonalität
Polyklonal
Konjugat
Dieser RFP Antikörper ist konjugiert mit Biotin
8
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
133
70
61
53
33
29
7
5
3
3
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
Isotyp IgG
Kreuzreaktivität (Details) Ads. to Hu, Ms, Rt Serum Proteins
Produktmerkmale Molar Ratio: 10-20 BAC molecules per IgG molecule
Reinigung Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Hintergrund Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
Gen-ID 86600
UniProt Q9U6Y8
Applikationshinweise Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used todetect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotinconjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP iswell suited to titrate RFP in solution. The detection antibody conjugated to biotin issubsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugatedpolyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cellexpression systems and can detect RFP containing inserts. Significant amplification ofsignal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to thefluorescence of RFP alone. For immunoblotting use either alkaline phosphatase orperoxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins onwestern blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 0.1 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-Red Fluorescent Protein (RFP) antibody (Biotin) (ABIN401226) anti-Red Fluorescent Protein (RFP) antibody (Biotin)
Allgemeine Veröffentlichungen Gross, Baird, Hoffman, Baldridge, Tsien: "The structure of the chromophore within DsRed, a red fluorescent protein from coral." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 22, pp. 11990-5, 2000 (PubMed).

Haben Sie etwas anderes gesucht?