anti-Human E2F4 Antikörper für ELISA

Recommended E2F4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antikörper
  • E2F-4
  • 2010111M04Rik
  • AI427446
  • E2F4
  • e2f-4
  • fb72f07
  • wu:fb72f07
  • wu:fe05f06
  • zgc:63815
  • E2F transcription factor 4
  • E2F transcription factor 4 S homeolog
  • E2F4
  • E2f4
  • e2f4.S
  • e2f4
Epitop
AA 211-301
9
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reaktivität
Human
93
41
39
7
6
5
5
5
5
3
3
2
1
Wirt
Maus
55
25
16
Klonalität
Monoklonal
Konjugat
Dieser E2F4 Antikörper ist unkonjugiert
5
4
3
2
2
2
Applikation
ELISA, Western Blotting (WB)
84
60
31
17
11
10
3
2
2
Optionen
Hersteller
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Bilder

Produktnummer ABIN394384
$ 461.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.448601 ABIN560681 ELISA WB Mouse IgG1 kappa AA 211-301, partial Anmelden zum Anzeigen 5B7 0
1 ABIN1533259 IHC ELISA WB Rabbit IgG AA 51-100 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1590021 ELISA WB Goat Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN1590023 ELISA WB Goat Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN2460194 ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN3181883 ELISA WB Rabbit IgG acLys96 Anmelden zum Anzeigen Polyclonal 0
1 ABIN3187838 ELISA IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1827793 ELISA WB Mouse IgG1, kappa AA 211-301 Anmelden zum Anzeigen 5B7 0
1 ABIN1978609 ELISA WB Goat AA 349-360 Anmelden zum Anzeigen Polyclonal 0
1 ABIN210875 IP ELISA WB Mouse IgG2b Anmelden zum Anzeigen 0
1 ABIN2562334 ELISA WB Goat IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN5687827 ELISA WB Goat Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN2889188 ELISA IHC (p) WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN5959438 ELISA WB Rabbit IgG acLys96 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5633732 ELISA WB Rabbit IgG AA 61-110, acLys96 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5633731 ELISA WB Rabbit IgG AA 61-110, Lys96 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5959439 ELISA IHC (p) WB Rabbit IgG Lys96 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2256942 ELISA WB Biotin Goat Anmelden zum Anzeigen Polyclonal 0
1 ABIN2256943 ELISA WB PE Goat Anmelden zum Anzeigen Polyclonal 0
1 ABIN2256948 ELISA WB APC Goat Anmelden zum Anzeigen Polyclonal 0

General

Antigen E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antikörper
Epitop AA 211-301
(9), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(93), (41), (39), (7), (6), (5), (5), (5), (5), (3), (3), (2), (1)
Wirt Maus
(55), (25), (16)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser E2F4 Antikörper ist unkonjugiert
(5), (4), (3), (2), (2), (2)
Applikation ELISA, Western Blotting (WB)
(84), (60), (31), (17), (11), (10), (3), (2), (2)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-E2F4 Antikörper

Target Details E2F4 Anwendungsinformationen Handhabung Referenzen für anti-E2F4 Antikörper (ABIN394384) Bilder
Spezifität E2F4 (NP_001941, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Kreuzreaktivität (Details) Human: ~~ Positive
Reinigung This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: E2F4 (NP_001941, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Klon 5B7
Isotyp IgG1 kappa
Plasmids, Primers & others

Target Details E2F4

Produktdetails anti-E2F4 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-E2F4 Antikörper (ABIN394384) Bilder zurück nach oben
Antigen
Andere Bezeichnung E2F4 (E2F4 Antibody Abstract)
Hintergrund

Other Names: Transcription factor E2F4, E2F-4, E2F4

Gene Description: E2F transcription factor 4, p107/p130-binding

Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq

Molekulargewicht 43960 DA
Gen-ID 1874
NCBI Accession NP_001941
UniProt Q16254
Pathways Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size

Anwendungsinformationen

Produktdetails anti-E2F4 Antikörper Target Details E2F4 Handhabung Referenzen für anti-E2F4 Antikörper (ABIN394384) Bilder zurück nach oben
Applikations-hinweise Western blot = 1:500-1000
Kommentare

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-E2F4 Antikörper Target Details E2F4 Anwendungsinformationen Referenzen für anti-E2F4 Antikörper (ABIN394384) Bilder zurück nach oben
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Haltbarkeit 6 months

Referenzen für anti-E2F4 Antikörper (ABIN394384)

Produktdetails anti-E2F4 Antikörper Target Details E2F4 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

van Amerongen, Diehl, Novoyatleva, Patra, Engel: "E2F4 is required for cardiomyocyte proliferation." in: Cardiovascular research, Vol. 86, Issue 1, pp. 92-102, 2010

Bankovic, Stojsic, Jovanovic, Andjelkovic, Milinkovic, Ruzdijic, Tanic: "Identification of genes associated with non-small-cell lung cancer promotion and progression." in: Lung cancer (Amsterdam, Netherlands), Vol. 67, Issue 2, pp. 151-9, 2010

Cunningham, Vierkant, Sellers, Phelan, Rider, Liebow, Schildkraut, Berchuck, Couch, Wang, Fridley, Gentry-Maharaj, Menon, Hogdall, Kjaer, Whittemore, DiCioccio, Song, Gayther, Ramus, Pharaoh, Goode: "Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis." in: British journal of cancer, Vol. 101, Issue 8, pp. 1461-8, 2009

Garneau, Paquin, Carrier, Rivard: "E2F4 expression is required for cell cycle progression of normal intestinal crypt cells and colorectal cancer cells." in: Journal of cellular physiology, Vol. 221, Issue 2, pp. 350-8, 2009

Lu, Dollé, Imholz, van t Slot, Verschuren, Wijmenga, Feskens, Boer: "Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations." in: Journal of lipid research, Vol. 49, Issue 12, pp. 2582-9, 2008

Bilder

Produktdetails anti-E2F4 Antikörper Target Details E2F4 Anwendungsinformationen Handhabung Referenzen für anti-E2F4 Antikörper (ABIN394384) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (AA 211-301) antibody (ABIN394384) E2F4 monoclonal antibody (M01), clone 5B7 Western Blot analysis of E2F4 expression in...
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (AA 211-301) antibody (ABIN394384) anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (AA 211-301) antibody (Image 2)
Western Blotting (WB) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (AA 211-301) antibody (ABIN394384) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
ELISA image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (AA 211-301) antibody (ABIN394384) Detection limit for recombinant GST tagged E2F4 is approximately 0.03ng/ml as a captu...