anti-Human TLR4 Antikörper für Immunofluorescence

Recommended TLR4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Toll-Like Receptor 4 (TLR4) Antikörper
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Epitop
AA 400-450, Internal Region
59
29
28
25
16
16
15
15
13
13
11
9
8
8
7
6
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
555
274
174
94
72
72
64
27
12
9
4
2
1
1
Wirt
Kaninchen
339
273
47
7
Klonalität
Polyklonal
Konjugat
Dieser TLR4 Antikörper ist unkonjugiert
49
47
47
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
392
340
185
136
129
123
98
92
34
31
27
26
26
21
20
16
14
8
7
4
4
3
2
1
1
1
Hersteller
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Bilder

Produktnummer ABIN252535
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN252522 ChIP CyTOF FACS ICC IF IHC IHC (fro) IHC (p) in vitro WB Mouse IgG2b kappa Anmelden zum Anzeigen 76B357-1 72
1 ABIN4360113 BP CyTOF FACS Func ICC IF IP in vitro Neut Mouse IgG2a Anmelden zum Anzeigen HTA125 45
1 ABIN4360167 BP CyTOF FACS ICC IF IHC (p) Neut WB Mouse IgG2b kappa Anmelden zum Anzeigen 76B357-1 24
1 ABIN4360119 BP CyTOF FACS ICC IF IP in vitro Neut Mouse IgG2a Anmelden zum Anzeigen HTA125 22
1 ABIN4360164 FACS ICC IF Alexa Fluor 488 Mouse IgG2a Anmelden zum Anzeigen HTA125 21
1 ABIN4360158 BP ELISA FACS ICC IF IP Neut Biotin Mouse IgG2a Anmelden zum Anzeigen HTA125 18
1 ABIN2485968 ICC FACS IF IHC WB Atto 700 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485967 ICC FACS IF IHC WB Atto 680 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485976 ICC FACS IF IHC WB PE Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485977 ICC FACS IF IHC WB Streptavidin Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485964 ICC FACS IF IHC WB Atto 594 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485962 ICC FACS IF IHC WB Atto 488 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485966 ICC FACS IF IHC WB Atto 655 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485961 ICC FACS IF IHC WB Atto 390 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485963 ICC FACS IF IHC WB Atto 565 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485965 ICC FACS IF IHC WB Atto 633 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485969 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485971 ICC FACS IF IHC WB Biotin Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485974 ICC FACS IF IHC WB PE-Atto 594 Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6
1 ABIN2485973 ICC FACS IF IHC WB HRP Rabbit AA 420-435 Anmelden zum Anzeigen Polyclonal 6

General

Antigen Toll-Like Receptor 4 (TLR4) Antikörper
Epitop AA 400-450, Internal Region
(59), (29), (28), (25), (16), (16), (15), (15), (13), (13), (11), (9), (8), (8), (7), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(555), (274), (174), (94), (72), (72), (64), (27), (12), (9), (4), (2), (1), (1)
Wirt Kaninchen
(339), (273), (47), (7)
Klonalität
Polyklonal
Konjugat Dieser TLR4 Antikörper ist unkonjugiert
(49), (47), (47), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(392), (340), (185), (136), (129), (123), (98), (92), (34), (31), (27), (26), (26), (21), (20), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1)
Pubmed 13 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TLR4 Antikörper

Target Details TLR4 Anwendungsinformationen Handhabung Referenzen für anti-TLR4 Antikörper (ABIN252535) Bilder
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the mouse TLR4 protein (between residues 400-450) [NP_612564]
Plasmids, Primers & others

Target Details TLR4

Produktdetails anti-TLR4 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR4 Antikörper (ABIN252535) Bilder zurück nach oben
Antigen
Andere Bezeichnung TLR4 (TLR4 Antibody Abstract)
Hintergrund Gene Symbol: TLR4
Molekulargewicht Theoretical MW: 75 kDa
Gen-ID 7099
UniProt Q9QUK6
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Handhabung Referenzen für anti-TLR4 Antikörper (ABIN252535) Bilder zurück nach oben
Applikations-hinweise Western Blot 1-3 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:50, Immunocytochemistry/Immunofluorescence 1:25-1:75, Immunohistochemistry-Paraffin 1:50This TLR4 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western Blot protocol specific for TLR4 antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Anwendungsinformationen Referenzen für anti-TLR4 Antikörper (ABIN252535) Bilder zurück nach oben
Format Liquid
Konzentration 1.12 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-TLR4 Antikörper (ABIN252535)

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Li, Kroin, Kc, Gibson, Chen, Corbett, Pahan, Fayyaz, Kim, van Wijnen, Suh, Kim, Im: "Altered spinal microRNA-146a and the microRNA-183 cluster contribute to osteoarthritic pain in knee joints." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 28, Issue 12, pp. 2512-22, 2013

Lu, Li, Samuvel, Jin, Zhang, Lopes-Virella, Huang et al.: "MD-2 is involved in the stimulation of matrix metalloproteinase-1 expression by interferon-γ and high glucose in mononuclear cells - a potential role of MD-2 in Toll-like receptor 4-independent ..." in: Immunology, Vol. 140, Issue 3, pp. 301-13, 2013 (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Flores-Espinosa, Pineda-Torres, Vega-Sánchez, Estrada-Gutiérrez, Espejel-Nuñez, Flores-Pliego, Maida-Claros, Paredes-Vivas, Morales-Méndez, Sosa-González, Chávez-Mendoza, Zaga-Clavellina: "Progesterone elicits an inhibitory effect upon LPS-induced innate immune response in pre-labor human amniotic epithelium." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 71, Issue 1, pp. 61-72, 2013 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Cammarota, Bertolini, Pennesi, Bucci, Gottardi, Garlanda, Laghi, Barberis, Sessa, Noonan, Albini: "The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker." in: Journal of translational medicine, Vol. 8, pp. 112, 2010 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Barajon, Serrao, Arnaboldi, Opizzi, Ripamonti, Balsari, Rumio: "Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 11, pp. 1013-23, 2009 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting,Immunohistochemistry

El Shikh, El Sayed, Wu, Szakal, Tew: "TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4444-50, 2007

Herber, Maloney, Roth, Freeman, Morgan, Gordon: "Diverse microglial responses after intrahippocampal administration of lipopolysaccharide." in: Glia, Vol. 53, Issue 4, pp. 382-91, 2006 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Carrithers, Tandon, Canosa, Michaud, Graesser, Madri: "Enhanced susceptibility to endotoxic shock and impaired STAT3 signaling in CD31-deficient mice." in: The American journal of pathology, Vol. 166, Issue 1, pp. 185-96, 2005 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting,Flow Cytometry

Allgemeine Veröffentlichungen

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001

Takeuchi, Hoshino, Kawai, Sanjo, Takada, Ogawa, Takeda, Akira: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999

Muzio, Natoli, Saccani, Levrero, Mantovani: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997

Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997

Bilder

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Anwendungsinformationen Handhabung Referenzen für anti-TLR4 Antikörper (ABIN252535) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Toll-Like Receptor 4 (TLR4) (AA 400-450), (Internal Region) antibody (ABIN252535) Western Blot: TLR4 Antibody [ABIN252535] - Western blot analysis of TLR4 using ABIN25...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 400-450), (Internal Region) antibody (ABIN252535) Immunohistochemistry: TLR4 Antibody [ABIN252535] - IHC analysis of TLR4 in formalin-f...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 400-450), (Internal Region) antibody (ABIN252535) Immunocytochemistry: TLR4 Antibody [ABIN252535] - ICC analysis of TLR4 in formalin-fi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) (AA 400-450), (Internal Region) antibody (ABIN252535) Immunohistochemistry-Paraffin: TLR4 Antibody [ABIN2525351] - Analysis of TLR4 in form...
Immunofluorescence (IF) image for anti-Toll-Like Receptor 4 (TLR4) (AA 400-450), (Internal Region) antibody (ABIN252535) Immunocytochemistry/Immunofluorescence: TLR4 Antibody [ABIN2525351] - TLR4 antibody w...