anti-Maus Aurora Kinase B Antikörper für Immunocytochemistry

Recommended Aurora Kinase B Antibody

Antigen
Aurora Kinase B (AURKB) Antikörper
  • AIK2
  • AIM-1
  • AIM1
  • ARK2
  • AurB
  • IPL1
  • PPP1R48
  • STK12
  • STK5
  • aurkb-sv1
  • aurkb-sv2
  • AIRK2
  • AL022959
  • Aik2
  • Aim1
  • Ark2
  • STK-1
  • Stk12
  • Stk5
  • Aur B
  • Aurora B
  • Aurora-B
  • CG6620
  • DmAIRK2
  • DmAurB
  • DmAuroraB
  • Dmel\\CG6620
  • IAL
  • IAL1
  • Ial
  • aurB
  • aurora B
  • ARK-2
  • XAIRK2
  • aik2
  • aim-1
  • aim1
  • airk2
  • ark2
  • ipl1
  • stk12
  • stk5
  • CHUNP6896
  • fa09g06
  • stka
  • wu:fa09g06
  • aurkb
  • AIRK2-B
  • XAIRK2-B
  • aurkb-a
  • aurkb-b
  • xAurora-B-B
  • aurora kinase B
  • aurora B
  • aurora kinase B S homeolog
  • AURKB
  • Aurkb
  • aurB
  • aurkb
  • aurkb.S
Epitop
pThr232
44
32
27
21
16
13
12
10
10
9
7
7
7
7
6
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Affe, Maus, Ratte (Rattus)
291
119
91
16
8
7
4
2
2
2
1
1
1
Wirt
Kaninchen
217
70
7
7
Klonalität
Polyklonal
Konjugat
Dieser Aurora Kinase B Antikörper ist unkonjugiert
12
9
9
8
8
7
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
231
142
106
92
43
32
15
11
11
10
10
8
2
2
1
Optionen

Bilder

Produktnummer ABIN6256516
$ 390.77
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
13 ABIN6260155 ELISA ICC IF WB Rabbit IgG Polyclonal 0
12.33321 ABIN151760 ICC IF IHC IHC (p) IP MA WB Rabbit C-Term Polyclonal 12
10 ABIN6260154 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
7 ABIN2668672 ICC IF WB Rabbit C-Term Polyclonal 0
1 ABIN2668035 ICC IF WB Rabbit Polyclonal 0
1 ABIN6582341 ICC IF IHC WB Rabbit pThr232 Polyclonal 0
1 ABIN6583165 ICC IF WB Rabbit Polyclonal 0
-4.16679 ABIN4282455 ICC IF WB Mouse IgG2a 3F11 0
-4.16679 ABIN4282457 ICC IF WB Mouse IgG1 6G2 0
-7.16679 ABIN5945106 ICC IF IHC IHC (p) IP WB Rabbit IgG N-Term SC55-08 0
-13.16679 ABIN4282454 ICC IF WB Mouse IgG2a 3F11 0
-13.16679 ABIN4282456 ICC IF WB Mouse IgG1 6G2 0
-13.16679 ABIN4282460 ICC IF WB Alexa Fluor 647 Mouse IgG2a 3F11 0
-13.16679 ABIN4282458 ICC IF WB Alexa Fluor 405 Mouse IgG2a 3F11 0
-13.16679 ABIN4282461 ICC IF WB Alexa Fluor 700 Mouse IgG2a 3F11 0
-13.16679 ABIN4282463 ICC IF WB Biotin Mouse IgG2a 3F11 0
-13.16679 ABIN4282464 ICC IF WB DyLight 650 Mouse IgG2a 3F11 0
-13.16679 ABIN4282465 ICC IF FITC Mouse IgG2a 3F11 0
-13.16679 ABIN4282466 ICC IF WB DyLight 680 Mouse IgG2a 3F11 0
-13.16679 ABIN4282467 ICC IF WB DyLight 488 Mouse IgG2a 3F11 0

General

Antigen Aurora Kinase B (AURKB) Antikörper
Epitop pThr232
(44), (32), (27), (21), (16), (13), (12), (10), (10), (9), (7), (7), (7), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Affe, Maus, Ratte (Rattus)
(291), (119), (91), (16), (8), (7), (4), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(217), (70), (7), (7)
Klonalität
Polyklonal
Konjugat Dieser Aurora Kinase B Antikörper ist unkonjugiert
(12), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(231), (142), (106), (92), (43), (32), (15), (11), (11), (10), (10), (8), (2), (2), (1)
Hersteller

Produktdetails anti-Aurora Kinase B Antikörper

Target Details Aurora Kinase B Anwendungsinformationen Handhabung Bilder
Spezifität Phospho-AurB (Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Threonine 232
Kreuzreaktivität Human, Affe, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human AurB around the phosphorylation site of Threonine 232
Isotyp IgG
Plasmids, Primers & others

Target Details Aurora Kinase B

Produktdetails anti-Aurora Kinase B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung AurB (AURKB Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Gene: AURKB

Molekulargewicht 39kDa
Gen-ID 9212
UniProt Q96GD4
Pathways T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-Aurora Kinase B Antikörper Target Details Aurora Kinase B Handhabung Bilder zurück nach oben
Applikations-hinweise WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Aurora Kinase B Antikörper Target Details Aurora Kinase B Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months

Bilder

Produktdetails anti-Aurora Kinase B Antikörper Target Details Aurora Kinase B Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) Western blot analysis of AurB phosphorylation expression in Nocodazole treated COS7 w...
Immunohistochemistry (IHC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 at 1/100 staining human Bone marrow tissue sections by IHC-P. The tissue ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 staining HT-1080 cells by ICC/IF. Cells were fixed with PFA and permeabil...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized...