Esel anti-Maus IgG (Heavy & Light Chain) Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6699038

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Esel

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Verwendungszweck: Donkey anti-Mouse IgG DyLight 800™ Conjugated Antibody

Spezifität: This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

Produktmerkmale:

Synonyms: Donkey anti-Mouse IgG DyLight 800™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 800™ Conjugation

Background: Anti-Mouse IgG DyLight800 Antibody generated in donkey detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.

Immunogen: Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 1.7

Anwendungsinformationen

Applikationshinweise:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: 1:10,000-1:25,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Holgate, Tarren, Bartlett: "Sex Specific Alterations in α4*Nicotinic Receptor Expression in the Nucleus Accumbens." in: Brain sciences, Vol. 8, Issue 4, (2018) (PubMed).

Newman, Schiavon, Turn, Kahn: "The ARL2 GTPase regulates mitochondrial fusion from the intermembrane space." in: Cellular logistics, Vol. 7, Issue 3, pp. e1340104, (2017) (PubMed).

Newman, Schiavon, Kahn: "Plasmids for variable expression of proteins targeted to the mitochondrial matrix or intermembrane space." in: Cellular logistics, Vol. 6, Issue 4, pp. e1247939, (2016) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody