Ziege anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper (HRP)
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Meerschweinchen
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- HRP
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Homologie
- guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins
- Produktmerkmale
- Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
- Aufreinigung
- Affinity purification
- Immunogen
- Purified Guinea pig IgG, whole molecule
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- Applikationshinweise
- 1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)
- Kommentare
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This antibody reacts with the heavey chaings on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Buffer
- 10 mM Sodium Phosphate, 0.15 M SodiumChloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
- Konservierungsmittel
- Kathon CG
- Handhabung
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
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