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Esel anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
Produktnummer ABIN1043955
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2441
    • 554
    • 469
    • 210
    • 133
    • 129
    • 96
    • 29
    • 28
    • 28
    • 20
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 727
    • 726
    • 605
    • 495
    • 391
    • 247
    • 213
    • 167
    • 138
    • 136
    • 132
    • 116
    • 107
    • 97
    • 94
    • 63
    • 49
    • 38
    • 29
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Meerschweinchen
    Wirt
    • 1837
    • 1575
    • 565
    • 223
    • 126
    • 124
    • 33
    • 32
    • 21
    • 20
    • 18
    • 15
    • 11
    • 1
    • 1
    Esel
    Klonalität
    • 3488
    • 193
    • 1
    Polyklonal
    Konjugat
    • 598
    • 420
    • 419
    • 395
    • 325
    • 279
    • 276
    • 121
    • 109
    • 97
    • 66
    • 66
    • 63
    • 62
    • 52
    • 51
    • 50
    • 46
    • 45
    • 44
    • 43
    • 39
    • 36
    • 34
    • 32
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 17
    • 17
    • 15
    • 14
    • 10
    Biotin
    Applikation
    • 2020
    • 1942
    • 1904
    • 1585
    • 1106
    • 934
    • 910
    • 529
    • 238
    • 115
    • 115
    • 111
    • 104
    • 78
    • 64
    • 56
    • 50
    • 50
    • 31
    • 28
    • 26
    • 21
    • 16
    • 15
    • 11
    • 9
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.
    Kreuzreaktivität
    Meerschweinchen
    Aufreinigung
    Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
    Immunogen

    Immunogen: Guinea Pig IgG whole molecule

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Centrifuge product if not completely clear after standing at room temperature.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Donkey Anti-Guinea Pig IgG Biotin Conjugation, Donkey Anti Guinea Pig IgG Biotin Conjugated

    Background: Anti-Guinea Pig IgG Biotin Antibody generated in donkey detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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