Ziege anti-Maus IgG3 (Heavy Chain) Antikörper - Preadsorbed

Produktnummer ABIN1043980

Produktdetails

Target: IgG3

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Kreuzreaktivität: Maus

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:2,000 - 1:10,000

Application Note: Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats.

ELISA Dilution: 1:40,000 - 1:200,000

Western Blot Dilution: 1:4,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG3

Abstract: IgG3 Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-MOUSE IgG3 Antibody, goat anti-MOUSE IgG3 (gamma 3) Antibody

Background: Anti-Mouse IgG3 Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.