Ziege anti-Maus IgG3 (Heavy Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN1118505

Produktdetails

Target: IgG3

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Produktmerkmale: HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Anti-Mouse IgG3 peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Referenzen

Guo, Kong, Peng, Long, Zhou, Shu: "Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5007-5014, (2017) (PubMed).

Chen, Ruan, Long, Peng, Zhou, Liu, Zhou, Liu et al.: "Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway ..." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2411-2416, (2017) (PubMed).

Antigendetails

Target: IgG3

Abstract: IgG3 Produkte

Substanzklasse: Antibody