anti-Maus GDNF Antikörper für Western Blotting

Recommended GDNF Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Glial Cell Line Derived Neurotrophic Factor (GDNF) Antikörper
  • ATF
  • BDPLT5
  • QPD
  • UPA
  • URK
  • u-PA
  • gdnf
  • GDNF
  • AI385739
  • gdnfa
  • hfb1-gdnf
  • ATF1
  • ATF2
  • HFB1-GDNF
  • HSCR3
  • plasminogen activator, urokinase
  • glial cell derived neurotrophic factor
  • glial cell derived neurotrophic factor a
  • glial cell line derived neurotrophic factor
  • glial cell derived neurotrophic factor L homeolog
  • PLAU
  • Gdnf
  • gdnfa
  • GDNF
  • gdnf.L
Epitop
AA 166-211
16
14
10
10
6
6
4
3
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
165
77
57
1
Wirt
Kaninchen
152
22
9
4
Klonalität
Polyklonal
Konjugat
Dieser GDNF Antikörper ist unkonjugiert
18
11
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
47
37
28
27
21
13
12
10
6
5
4
4
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN736536
$ 318.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.061829 ABIN652624 FACS IHC (p) WB Rabbit Ig Fraction AA 8-36, N-Term Anmelden zum Anzeigen Polyclonal 1
3.061829 ABIN453664 EIA WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 1
3.061829 ABIN1172051 ICC IHC WB Rabbit IgG AA 79-217 Anmelden zum Anzeigen Polyclonal 0
3.061829 ABIN5948923 FACS WB Rabbit IgG AA 50-150 Anmelden zum Anzeigen JA93-10 0
3.061829 ABIN3031042 FACS IHC ELISA WB Rabbit Ig Fraction AA 8-36 Anmelden zum Anzeigen Polyclonal 0
3.061829 ABIN2930742 IF/ICC IHC IP WB Rabbit AA 79-217 Anmelden zum Anzeigen Polyclonal 0
3.061829 ABIN3003865 FACS IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.061829 ABIN3031043 ICC IHC (p) WB Rabbit IgG Middle Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN223529 IP IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN790851 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN6280609 ELISA WB Rabbit IgG AA 100-180 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1002352 IP IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN2621281 ICC IHC IHC (p) WB Rabbit IgG AA 142-158 Anmelden zum Anzeigen Polyclonal 0
1 ABIN736545 IHC (p) WB HRP Rabbit IgG AA 166-211 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2603551 WB Rabbit IgG AA 79-217 Anmelden zum Anzeigen Polyclonal 0
1 ABIN736538 IHC (p) WB Biotin Rabbit IgG AA 166-211 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1898140 ELISA WB APC Rabbit IgG AA 8-36 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1898139 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 8-36 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1898141 ELISA WB FITC Rabbit IgG AA 8-36 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1898142 ELISA WB PE Rabbit IgG AA 8-36 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Glial Cell Line Derived Neurotrophic Factor (GDNF) Antikörper
Epitop AA 166-211
(16), (14), (10), (10), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(165), (77), (57), (1)
Wirt Kaninchen
(152), (22), (9), (4)
Klonalität
Polyklonal
Konjugat Dieser GDNF Antikörper ist unkonjugiert
(18), (11), (7), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(172), (47), (37), (28), (27), (21), (13), (12), (10), (6), (5), (4), (4), (2), (2), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-GDNF Antikörper

Target Details GDNF Anwendungsinformationen Handhabung Referenzen für anti-GDNF Antikörper (ABIN736536) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human GDNF
Isotyp IgG
Plasmids, Primers & others

Target Details GDNF

Produktdetails anti-GDNF Antikörper Anwendungsinformationen Handhabung Referenzen für anti-GDNF Antikörper (ABIN736536) Bilder zurück nach oben
Antigen
Andere Bezeichnung GDNF (GDNF Antibody Abstract)
Hintergrund

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular location: Secreted

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

Gen-ID 2668
UniProt P39905
Pathways RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails anti-GDNF Antikörper Target Details GDNF Handhabung Referenzen für anti-GDNF Antikörper (ABIN736536) Bilder zurück nach oben
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-GDNF Antikörper Target Details GDNF Anwendungsinformationen Referenzen für anti-GDNF Antikörper (ABIN736536) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-GDNF Antikörper (ABIN736536)

Produktdetails anti-GDNF Antikörper Target Details GDNF Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, 2014 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: Brain

Bilder

Produktdetails anti-GDNF Antikörper Target Details GDNF Anwendungsinformationen Handhabung Referenzen für anti-GDNF Antikörper (ABIN736536) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyc...
SDS-PAGE (SDS) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal ...
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Poly...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
'Independent Validation' Siegel
Antigen GDNF
Chargennummer 9F01M8
Validierte Anwendung Immunofluorescence
Positivkontrolle mouse hippocampus and putamen
Negativkontrolle no primary control
Bewertung

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Primärantikörper ABIN736536
Sekundärantikörper anti-rabbit AF488 conjugated antibody (Life Technologies)
Protokoll
  • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
  • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
  • Wash spinal cord and brain blocks several times with PBS.
  • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
  • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
  • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
  • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Anmerkungen
  • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

  • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

Validierungsbilder
Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536)

IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal hor...