GDNF Antikörper (Middle Region)

Produktnummer ABIN3044406

Produktdetails anti-GDNF Antikörper

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Bindungsspezifität: AA 142-158, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: FRYCSGSCDA AETTYDK

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: glial cell derived neurotrophic factor
Protein Name: Glial cell line-derived neurotrophic factor(hGDNF)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-GDNF Antikörper (ABIN3044406)

A, Xu, Zhao, Li, Qi, Li, Wang, Zhou: "Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells." in: Biomedical engineering online, Vol. 17, Issue 1, pp. 88, (2018) (PubMed).

Zhang, Shi, Li, Zhang, Hao: "Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 87-93, (2014) (PubMed).

Liu, Wang, Liu, Yang, Xu, Ren: "Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction." in: Neural regeneration research, Vol. 8, Issue 23, pp. 2190-7, (2014) (PubMed).

Li, Xia, Zhang, Wu: "S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk." in: PLoS ONE, Vol. 6, Issue 6, pp. e21663, (2011) (PubMed).

Details zu GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: GDNF (GDNF Produkte)

Synonyme: gdnf antikoerper, GDNF antikoerper, AI385739 antikoerper, gdnfa antikoerper, hfb1-gdnf antikoerper, ATF1 antikoerper, ATF2 antikoerper, HFB1-GDNF antikoerper, HSCR3 antikoerper, glial cell derived neurotrophic factor antikoerper, glial cell derived neurotrophic factor a antikoerper, glial cell line derived neurotrophic factor antikoerper, glial cell derived neurotrophic factor L homeolog antikoerper, Gdnf antikoerper, gdnfa antikoerper, GDNF antikoerper, gdnf.L antikoerper

Hintergrund: GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.

Synonyms: gdnf antibody|GDNF_HUMAN antibody|Glial cell derived neurotrophic factor antibody|Glial Cell Line Derived Neurotrophic Factor antibody|Glial cell line-derived neurotrophic factor antibody|Glial derived neurotrophic factor antibody|HFB1 GDNF antibody|hGDNF antibody|HSCR3 antibody

UniProt: P39905

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration