Vitronectin Protein (VTN) (His tag)

Produktnummer ABIN7536595

Produktdetails

Target: Vitronectin (VTN)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Vitronectin Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Vitronectin/V75/VTN Protein

Sequenz: DQESCKGRCT QGFMASKKCQ CDELCTYYQS CCADYMEQCK PQVTRGDVFT MPEDDYWSYD YVEEPKNNTN TGVQPENTSP PGDLNPRTDG TLKPTAFLDP EEQPSTPAPK VEQQEEILRP DTTDQGTPEF PEEELCSGKP FDAFTDLKNG SLFAFRGQYC YELDETAVRP GYPKLIQDVW GIEGPIDAAF TRINCQGKTY LFKGSQYWRF EDGVLDPGYP RNISEGFSGI PDNVDAAFAL PAHRYSGRER VYFFKGKQYW EYEFQQQPSQ EECEGSSLSA VFEHFALLQR DSWENIFELL FWGRSSDGAR EPQFISRNWH GVPGKVDAAM AGRIYVTGSL SHSAQAKKQK SKRRSRKRYR SRRGRGHRRS QSSNSRRSSR SIWFSLFSSE ESGLGTYNNY DYDMDWLVPA TCEPIQSVYF FSGDKYYRVN LRTRRVDSVN PPYPRSIAQY WLGCPTSEK

Spezifität: Asp20-Lys478

Reinheit: > 85 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: <1EU/μg

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37 °C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Konzentration: 1.45 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Antigendetails

Target: Vitronectin (VTN)

Andere Bezeichnung: Vitronectin/V75/VTN (VTN Produkte)

Synonyme: VTN Protein, DKFZp470F0511 Protein, LOC100228142 Protein, vtn Protein, zgc:112277 Protein, V75 Protein, VN Protein, VNT Protein, AI256434 Protein, Vn Protein, Aa1018 Protein, vitronectin Protein, vitronectin a Protein, vitronectin b Protein, vitronectin L homeolog Protein, VTN Protein, vtn Protein, vtna Protein, vtnb Protein, CpipJ_CPIJ016691 Protein, vtn.L Protein, Vtn Protein

Hintergrund:

Description: Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.

Name: V75, VN, VNT,VTN,VN,VNT

Gen-ID: 22370

UniProt: P29788

Pathways: Autophagie, Smooth Muscle Cell Migration