Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.The IC50 value is <0.19 nM.
Crystallography grade
SERPIND1
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
SERPIND1
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Buffer
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris,150 mM NaCl, pH 7.5.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Target
SERPIND1
(serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))