SERPIND1 Protein (His tag)

Produktnummer ABIN7197974

Produktdetails

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SERPIND1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse SerpinD1/HCF2 Protein (His Tag)(Active)

Sequenz: Met1-Ser 478

Produktmerkmale: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.

Reinheit: > 98 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catslog# ES011). The IC50 value is < 1.2 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM HEPES, 150 mM NaCl, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Andere Bezeichnung: SerpinD1/HCF2 (SERPIND1 Produkte)

Synonyme: D22S673 Protein, HC2 Protein, HCF2 Protein, HCII Protein, HLS2 Protein, LS2 Protein, THPH10 Protein, serpind1 Protein, MGC53936 Protein, rls2var1 Protein, AA985900 Protein, AI303446 Protein, Hcf2 Protein, serpin family D member 1 Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade D, member 1 Protein, SERPIND1 Protein, serpind1.L Protein, Serpind1 Protein, serpind1 Protein

Hintergrund:

Background: SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Synonym: Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1

Molekulargewicht: 53.5 kDa

NCBI Accession: NP_032249