SERPINA3 Protein (His tag)

Produktnummer ABIN7534222

Produktdetails

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SERPINA3 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein

Sequenz: NSPLDEENLT QENQDRGTHV DLGLASANVD FAFSLYKQLV LKAPDKNVIF SPLSISTALA FLSLGAHNTT LTEILKGLKF NLTETSEAEI HQSFQHLLRT LNQSSDELQL SMGNAMFVKE QLSLLDRFTE DAKRLYGSEA FATDFQDSAA AKKLINDYVK NGTRGKITDL IKDLDSQTMM VLVNYIFFKA KWEMPFDPQD THQSRFYLSK KKWVMVPMMS LHHLTIPYFR DEELSCTVVE LKYTGNASAL FILPDQDKME EVEAMLLPET LKRWRDSLEF REIGELYLPK FSISRDYNLN DILLQLGIEE AFTSKADLSG ITGARNLAVS QVVHKAVLDV FEEGTEASAA TAVKITLLSA LVETRTIVRF NRPFLMIIVP TDTQNIFFMS KVTNPKQA

Spezifität: Asn26-Ala423

Reinheit: > 97 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <11.5 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES,150 mM NaCl, pH 8.0.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Andere Bezeichnung: Serpin A3/Alpha-1-antichymotrypsin (SERPINA3 Produkte)

Synonyme: SERPINA3-1 Protein, Pi2 Protein, AACT Protein, ACT Protein, GIG25 Protein, A1AT Protein, PI Protein, alpha-1-PI Protein, AAT Protein, Pi Protein, Spi1 Protein, SERPINA3-5 Protein, serpina3 Protein, serpin A3-5 Protein, serpin family A member 3 Protein, alpha-1-antiproteinase F precursor Protein, serpin family A member 1 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog Protein, LOC396684 Protein, SERPINA3 Protein, LOC100008973 Protein, SERPINA1 Protein, Serpina1 Protein, SPIA5 Protein, serpina3.L Protein

Hintergrund:

Description: SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors. It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Serpin A3 is a major constituent of the plaques associated with Alzheimer's disease and an inhibitor of amyloid beta peptide degradation.

Name: AACT, ACT, GIG24, GIG25,SERPINA3,ACT,GIG24,GIG25

Gen-ID: 12

UniProt: P01011