Leptin Receptor Protein (LEPR) (Fc Tag,His tag)

Produktnummer ABIN7535076

Produktdetails

Target: Leptin Receptor (LEPR)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Leptin Receptor Protein ist gelabelt mit Fc Tag,His tag.

Verwendungszweck: Active Recombinant Human Leptin receptor/LEP-R/CD295 Protein

Sequenz: TAFNLSYPIT PWRFKLSCMP PNSTYDYFLL PAGLSKNTSN SNGHYETAVE PKFNSSGTHF SNLSKTTFHC CFRSEQDRNC SLCADNIEGK TFVSTVNSLV FQQIDANWNI QCWLKGDLKL FICYVESLFK NLFRNYNYKV HLLYVLPEVL EDSPLVPQKG SFQMVHCNCS VHECCECLVP VPTAKLNDTL LMCLKITSGG VIFQSPLMSV QPINMVKPDP PLGLHMEITD DGNLKISWSS PPLVPFPLQY QVKYSENSTT VIREADKIVS ATSLLVDSIL PGSSYEVQVR GKRLDGPGIW SDWSTPRVFT TQDVIYFPPK ILTSVGSNVS FHCIYKKENK IVPSKEIVWW MNLAEKIPQS QYDVVSDHVS KVTFFNLNET KPRGKFTYDA VYCCNEHECH HRYAELYVID VNINISCETD GYLTKMTCRW STSTIQSLAE STLQLRYHRS SLYCSDIPSI HPISEPKDCY LQSDGFYECI FQPIFLLSGY TMWIRINHSL GSLDSPPTCV LPDSVVKPLP PSSVKAEITI NIGLLKISWE KPVFPENNLQ FQIRYGLSGK EVQWKMYEVY DAKSKSVSLP VPDLCAVYAV QVRCKRLDGL GYWSNWSNPA YTVVMDIKVP MRGPEFWRII NGDTMKKEKN VTLLWKPLMK NDSLCSVQRY VINHHTSCNG TWSEDVGNHT KFTFLWTEQA HTVTVLAINS IGASVANFNL TFSWPMSKVN IVQSLSAYPL NSSCVIVSWI LSPSDYKLMY FIIEWKNLNE DGEIKWLRIS SSVKKYYIHD HFIPIEKYQF SLYPIFMEGV GKPKIINSFT QDDIEKHQSD

Spezifität: Thr20-Asp839

Reinheit: > 95 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein,the EC₅₀ of Leptin R/CD295 is 261.45 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl,1 mM DTT, pH 6.5.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: Leptin receptor/LEP-R/CD295 (LEPR Produkte)

Synonyme: CD295 Protein, LEP-R Protein, LEPRD Protein, OB-R Protein, OBR Protein, obr Protein, cd295 Protein, ob-rb Protein, Fa Protein, LEPROT Protein, Leprb Protein, Modb1 Protein, OB-RGRP Protein, Obr Protein, db Protein, diabetes Protein, obese-like Protein, obl Protein, ObRb Protein, Ob-R Protein, Ob-RM Protein, lepr Protein, leptin receptor Protein, leptin receptor S homeolog Protein, LEPR Protein, lepr Protein, Lepr Protein, lepr.S Protein

Hintergrund: CD295,LEP-R,LEPRD,OB-R,OBR,LEPR

Gen-ID: 3953

UniProt: P48357

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour