IGFBP4 Protein (His tag)

Produktnummer ABIN7520090

Produktdetails

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IGFBP4 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human IGFBP-4 Protein

Sequenz: MLPLCLVAAL LLAAGPGPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE PGCGCCATCA LGLGMPCGVY TPRCGSGLRC YPPRGVEKPL HTLMHGQGVC MELAEIEAIQ ESLQPSDKDE GDHPNNSFSP CSAHDRRCLQ KHFAKIRDRS TSGGKMKVNG APREDARPVP QGSCQSELHR ALERLAASQS RTHEDLYIIP IPNCDRNGNF HPKQCHPALD GQRGKCWCVD RKTGVKLPGG LEPKGELDCH QLADSFRE

Spezifität: Met1-Glu258

Reinheit: > 92 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 1.95-111.32 ng/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 1.95-122.3ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Andere Bezeichnung: IGFBP-4 (IGFBP4 Produkte)

Synonyme: IGFBP4 Protein, igfbp4 Protein, BP-4 Protein, HT29-IGFBP Protein, IBP4 Protein, IGFBP-4 Protein, AI875747 Protein, Deb2 Protein, IGF-BP4 Protein, IBP-4 Protein, insulin like growth factor binding protein 4 Protein, insulin-like growth factor-binding protein 4 Protein, insulin-like growth factor binding protein 4 Protein, IGFBP4 Protein, LOC100136305 Protein, LOC100194498 Protein, igfbp4 Protein, Igfbp4 Protein

Hintergrund:

Description: IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins . IGFBP-4 binds both insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. It circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. There is mounting evidence that IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

Name: IGFBP4,BP-4,HT29-IGFBP,IBP4,IGFBP-4

Gen-ID: 3487

UniProt: P22692

Pathways: WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process