Dieses R-Spondin 3 Protein ist gelabelt mit His tag.
Verwendungszweck
Human R spondin 3/RSPO3 Protein
Sequenz
Gln22-Val146
Produktmerkmale
Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val146.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
RSPO3
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Molekulargewicht
14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.