R-Spondin 3 Protein (RSPO3) (AA 1-146) (His tag)

Produktnummer ABIN7317631

Produktdetails

Target: R-Spondin 3 (RSPO3)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-146

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses R-Spondin 3 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human RSPO3 Protein (aa 1-146, His Tag)(Active)

Sequenz: Met 1-Val 146

Produktmerkmale: A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 96 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.2. Immobilized RSPO3-His (146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc .The EC50 of human RNF43-Fc is 0.01-0.03μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: R-Spondin 3 (RSPO3)

Andere Bezeichnung: RSPO3 (RSPO3 Produkte)

Synonyme: pwtsr Protein, thsd2 Protein, cristin1 Protein, CRISTIN1 Protein, PWTSR Protein, THSD2 Protein, RGD1563246 Protein, 2810459H04Rik Protein, AW742308 Protein, Cristin1 Protein, Thsd2 Protein, R-spondin 3 Protein, rspo3 Protein, RSPO3 Protein, Rspo3 Protein

Hintergrund:

Background: R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

Synonym: R-spondin-3,RSPO3,Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2, CRISTIN1

Molekulargewicht: 15.3 kDa