CLEC4C Protein (AA 48-212) (mFc Tag)

Produktnummer ABIN7490221

Produktdetails

Target: CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))

Protein-Typ: Recombinant

Proteineigenschaft: AA 48-212

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CLEC4C Protein ist gelabelt mit mFc Tag.

Verwendungszweck: Cynomolgus BDCA-2 Protein

Sequenz: Tyr48-Ile212

Produktmerkmale: Recombinant Cynomolgus BDCA-2 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Tyr48-Ile212.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus BDCA-2, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))

Andere Bezeichnung: BDCA-2 (CLEC4C Produkte)

Synonyme: BDCA2 Protein, CD303 Protein, CLECSF11 Protein, CLECSF7 Protein, DLEC Protein, HECL Protein, PRO34150 Protein, Clec4c Protein, C-type lectin domain family 4 member C Protein, CLEC4C Protein, LOC100733227 Protein

Hintergrund: BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.

Molekulargewicht: 46.00 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5UWP4